Krider, E. S.; Rack, J. J.; Frank, N. L. and Meade*, T. J. Automated Synthesis of 3'-Metalated Oligonucleotides Inorg. Chem. 2001 40, 4002-4009. (JUL 30 2001) DOI: 10.1021/ic010396v.
A novel strategy for generating site-specific metallated oligonucleotides for investigation of metal-to-metal charge transfer interactions in functionalized DNA is reported. A solid support for oligonucleotide synthesis containing [Ru(bpy)(2)(impy ')](2+) (bpy is 2.2 ' -bipyridine; impy ' is 2 ' -iminomethylpyridyl-2 ' -deoxyuridine) leads to SS DNA synthesis in high yield. While incorporation into the oligonucleotide does not result in changes in redox potentials or excited state chemistry of the metal complex, metallation of SS DNA leads to increased stability of the resulting duplexes.